There has been much debate on whether or not it is possible to determine the age of the blood found at 29 Violette Ave. Many want to know when the large amount of blood that was determined to be Ayla's was found by Justin's bed. Justin and his supporters want us to believe that it is irrelevant, that it was only a few spots of blood and that it was from a cut on Ayla's foot that did not happen the night before she was reported missing. In my opinion, we don't really know when Ayla really went missing. Justin may claim that it was the night of December 16th but he has shown time and time again that his word has to be taken with a grain of salt, or should I say Heidi and Angela's words need to be taken with a grain of salt, since Justin apparently only speaks through them.
The blood may be older than the 16th but it still wouldn't mean that it wasn't related to Ayla's disappearance. That being said, if we could determine when the blood was really from we may be able to pinpoint when Ayla really did disappear. The amount of blood is significant. A small child does not lose that much blood and not require in the very least stitches. More than 8 oz of blood loss in a toddler that doesn't weigh more than 25 lbs is more than what a cut on her foot would produce. The attempts to minimize the blood evidence from Justin supporters is sickening.
I have done some research on the possibility of determing how old blood is through different scientific methods. I am not a scientist nor am I a forensic scientist but I am capable of reading and comprehending, or at least I like to think I am anyway. I apologize if my interpretation of the articles I read are not accurate and since I am sure there are many people out there that enjoy pointing out when I am wrong, I can only imagine if I am, they will flood the comment section with corrections.
Scientists have been researching how to do this for many years. The ability to determine how old blood is would help investigators tremendously. As of now, the only way to really determine how old blood is, is in a lab. There is no instrument that can be taken to the crime scene. There is also no way to pinpoint the blood to a specific date, in this case where it was known that Ayla was only at the Violette Ave. residence for a relatively short period of time, it may not be possible to pinpoint when in this two month span the blood really was from other than that it was within the last two months and not from two years ago for example.
How The Age Of Blood Has Been Determined
Several approaches have been used in an attempt to determine the age of a bloodstain or other
biological material. The majority of methods rely on the transformation of hemoglobin into its
derivatives, and the changes in color and solubility which accompany them. Fiori (1962)
summarized a method that correlated the age of the bloodstain with the progressive diffusion of C1-
around the stain, which can be fixed as AgCl. Upon reduction, a black border will form around
stains which are more than two months old. The size of the border will increase in increments up to
nine months, indicating an approximate age of sample. Enzyme assays have also been used with
limited success. In general, they allow for a statement that the sample is either less than or more
than a certain age. Schwarz (1936) examined "peroxidase" in bloodstains as a method of
determining age. The peroxidase was shown to be a direct indication of the amount of hemoglobin
remaining in the stain. Guaiacum was used as a substrate for peroxidase, which produced a blue
color in the presence of hemoglobin. These studies suggested that the intensity of the blue color
varied with age. Another more recent approach (Rajamannarr, 1977) looked at the serum protein
profile by immunoelectrophoresis in stains as a function of their age from 15 days to one year. A
characteristic pattern of disappearance of various proteins at test points along the time line was
constructed. All of the proteins were undetectable at 365 days. In contradiction, Sensabaugh, 1971,
found albumin to be detectable by its immunological reaction in a dried blood sample eight years
old. This contradiction has been explained by a change in electrophoretic mobility of albumin in
aging bloodstains. The above approaches are incapable of discriminating blood samples from
different species, provide too narrow or unreliable a window of time to be of much use, are limited
to bloodstains and could have misleading results due to sample size.
One major issue with using any of the above methods is that they need to be done in a lab and in order for that to happen, there needs to be enough bloodstain samples to determine first and foremost, whose blood it is and then if there are any samples left and enough of a sample they can start the process of determining how old the blood is. Because the blood was found in a basement bedroom, the bloodstain is likely on concrete and from statements made by people claiming to see the spots circled in the house after police left, it does not appear LE removed parts of the concrete floor and sent them to a lab to determine the age of the bloodstains. These methods are also time consuming and because of the inability to determine an exact time and where we know Ayla was only at the DiPietro's for a small window of time, it is unlikely to determine anything other than that the blood is from October 17th to December 17th. LE was probably made aware of this and it is unlikely that we will ever really know when Ayla's blood was spilled in the basement bedroom.
There are new methods of trying to pinpoint the age of blood that are in the works now and hopefully these methods will be readily available to forensic scientists soon. One organization is working on a handheld instrument that can be brought into the field and can measure the spectra reflected from a stain and use precalibrated data to determine the age. Another group is working on determining the age of blood using Carbon 14 dating and RNA cells and although they have made strides, neither is anywhere near being used at this time for criminal cases or in court.
So, as much as we would all like to know exactly when Ayla's blood was spilled while she was in her father's care, we are not going to be able to rely on forensics for this information. We also have little to no chance that any of the DiPietro's are going to grow a conscience and tell the whole truth about what happened to Ayla while she was living there. We do know that Ayla sustained multiple injuries while in her father's care and as if a broken arm wasn't serious enough, we all are left wondering how could it be so much worse that she lost over 8 oz. of blood? We can only hope that if someone that knows the truth won't come forward and speak for Ayla that as the weather is warmer here in Maine and Spring has finally made it's appearance that Ayla will be found. Areas that were previously unaccessible due to snow are now cleared. The clock is ticking Elisha and Courtney. Make a deal before it is too late because we will find Ayla and once we do, you all will be going to jail and your children won't be your responsibility anymore. You will lose your right to make decisions for them, someone else will get to revel in their firsts, they will learn to call someone else Mommy, you will not be able to comfort them when they are sick or see them beam with happiness when they accomplish something. You will lose what you took away from Trista. You know what they say about Karma....